Analysis of the tetraspanin CD9–integrin αIIbβ3 (GPIIb-IIIa) complex in platelet membranes and transfected cells
نویسندگان
چکیده
The platelet integrin, α IIb β $ (GPIIb-IIIa), and the tetraspanin, CD9, are integral membrane proteins that are abundant in platelet membranes. We have identified several proteins, including CD9, which were co-precipitated by anti-α IIb β $ antibody from untreated, resting platelets that were solubilized with the poly(oxyethylene) non-ionic detergent, Brij-35. Immunoblot and quantitative immunoprecipitation showed that the association of α IIb β $ with CD9 is specific and stoichiometric. The interaction between CD9 and α IIb β $ is probably hydrophobic, as Triton X100 and hydrophobic detergents of the Brij series completely
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